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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been observed in lots of tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a important position in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not yet readily available.

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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically includes the epitope acknowledged via the antibody.

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This guidebook outlines more possible optimization of mobile and immunoassay parameters to make certain the very best effects are...

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This tutorial outlines more attainable optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...

Antibodies bound to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is specific.

Goal Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation designs which contribute to your temporal coordination of each mitotic celebration. Cyclin E sorts a posh with and functions for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells development by way of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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